Application of Green Fluorescent Protein to Affinity Electrophoresis; Affinity of IgG-Binding Domain C from Streptococcal Protein G to Mouse IgG1.

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  • Kazama Hitoshi
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido
  • Yamada Keiko
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido
  • Aoki Takashi
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido
  • Watabe Hiroyuki
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido

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Affinity electrophoresis (AEP) using green fluorescent protein (GFP) was studied. We constructed a fusion protein that linked S147PGFP and IgG binding domain C from streptococcal protein G (GFP–SpGC). The affinity of GFP–SpGC for mouse IgG1 was measured. The AEP using GFP does not require a staining step after electrophoresis, and was successful with a non-purified sample. Therefore, this method is simple and useful for measuring many samples such as those used in mutational studies.

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