Casein Kinase 2 (CK2)-Mediated Reduction of the Activities of Src Family Tyrosine Kinases in Vitro
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- Yokoyama Takamasa
- Genetical Biochemistry and Signal Biology, Graduate School of Medical Sciences, Kitasato University
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- Kamata Yayoi
- Genetical Biochemistry and Signal Biology, Graduate School of Medical Sciences, Kitasato University
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- Ohtsuki Kenzo
- Genetical Biochemistry and Signal Biology, Graduate School of Medical Sciences, Kitasato University
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説明
The physiological correlation between casein kinase 2 (CK2) and two Src family tyrosine kinases (Src-TKs, Fyn and Src) was mainly investigated in vitro. It was found that (i) Thr-residues of these two Src-TKs were preferentially phosphorylated by CK2 using [γ-32P]GTP as a phosphate donor; (ii) this phosphorylation was highly stimulated in the presence of poly-Arg; (iii) full phosphorylation of two Src-TKs by CK2 resulted in significant reduction of their TK activities; and (iv) quercetin (a CK2 inhibitor) inhibited the CK2-mediated reduction of their Src-TK activities in vitro. Under the same experimental conditions, similar results were obtained with Yes. These results suggest that CK2 may be a protein kinase responsible for the suppression of at least three Src-TKs (Fyn, Src and Yes) through the specific phosphorylation of their Thr-residues at the cellular level.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 27 (12), 1895-1899, 2004
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204627485568
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- NII論文ID
- 110003665729
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- NII書誌ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 7172417
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- PubMed
- 15577202
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可