Application of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Discrimination of Escherichia Strains Possessing Highly Conserved Ribosomal RNA Gene Sequences
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- Muroi Masashi
- Research Institute of Pharmaceutical Sciences, Musashino University
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- Shima Keisuke
- Analytical & Measuring Instruments Division, Shimadzu Corporation
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- Nakagawa Yasuyoshi
- Resource Collection Division, National Institute of Technology and Evaluation
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- Tanamoto Ken-ichi
- Research Institute of Pharmaceutical Sciences, Musashino University
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Abstract
We evaluated the capability of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to discriminate twelve Escherichia strains: E. blattae, E. fergusonii, E. hermanii and nine E. coli, whose ribosomal RNA (rRNA) gene sequence homologies are in the range of 96—100%. Similarities obtained by MALDI-TOF MS were found to be 78—92% among the E. coli strains, and 74% between E. coli and E. fergusonii. E. blattae and E. hermanii showed only 32% similarity when compared to the other species. Thus, MALDI-TOF MS provides capability of distinguishing bacterial species or even strains possessing highly conserved rRNA gene sequences.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 34 (3), 430-432, 2011
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390001204627499136
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- NII Article ID
- 130000657825
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- NII Book ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 10990185
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed