Biochemical Characterization of Suramin as a Selective Inhibitor for the PKA-Mediated Phosphorylation of HBV Core Protein in Vitro

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  • Okabe Motohito
    Genetical Biochemistry, School of Allied Health Sciences, Kitasato University
  • Enomoto Masato
    Genetical Biochemistry and Signal Biology, Graduate School of Medical Sciences, Kitasato University
  • Maeda Haruki
    Genetical Biochemistry and Signal Biology, Graduate School of Medical Sciences, Kitasato University
  • Kuroki Kazuyuki
    Division of Cell Biology, Cancer Research Institute, Kanazawa University
  • Ohtsuki Kenzo
    Genetical Biochemistry and Signal Biology, Graduate School of Medical Sciences, Kitasato University

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The inhibitory effect of suramin on the phosphorylation of GST-HBV core fusion protein (GST-Hcore) and two GST-Hcore fusion polypeptides (Hcore157B and Hcore164B) by two α-type cAMP-dependent protein kinases (PKAIα and PKAIIα) was biochemically investigated in vitro. It was found that (i) this phosphorylation was inhibited by suramin at a low concentration (IC50=approx. 10 nM); (ii) a relative high dose of suramin was required to inhibit an autophosphorylation of PKAIIα (IC50=approx. 0.7 μM) and the PKAIIα-mediated phosphorylation of histone H2B (IC50=approx. 0.4 μM); (iii) the PKAIIα-mediated phosphorylation of Hcore157B was more sensitive to suramin than the phosphorylation of Hcore157B by Ca2+-dependent protein kinase (PKC); and (iv) suramin had a high binding affinity for Hcore157B, but not for histone H2B in vitro. These results suggest that suramin selectively inhibits the PKA-mediated phosphorylation of HBV-CP through the direct binding in vitro of suramin to the Arg-rich C-terminal region (containing three potential phosphorylation sites for PKA) on HBV-CP.

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