Specific Inhibition of Hypoxia-inducible Factor (HIF)-1.ALPHA. Activation and of Vascular Endothelial Growth Factor (VEGF) Production by Flavonoids
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- Hasebe Yuki
- Department of Microbiology, Showa University of Pharmaceutical Sciences
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- Egawa Kiyoshi
- Department of Microbiology, Showa University of Pharmaceutical Sciences
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- Yamazaki Yoko
- Institute of Microbial Chemistry, Tokyo
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- Kunimoto Setsuko
- Institute of Microbial Chemistry, Tokyo
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- Hirai Yasuaki
- Department of Pharmacognosy and Phytochemistry, Showa University of Pharmaceutical Sciences
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- Ida Yoshiteru
- Department of Pharmacognosy and Phytochemistry, Showa University of Pharmaceutical Sciences
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- Nose Kiyoshi
- Department of Microbiology, Showa University of Pharmaceutical Sciences
Bibliographic Information
- Other Title
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- Specific Inhibition of Hypoxia-inducible Factor (HIF)-1α Activation and of Vascular Endothelial Growth Factor (VEGF) Production by Flavonoids
- Specific Inhibition of Hypoxia inducible Factor HIF 1 アルファ Activation and of Vascular Endothelial Growth Factor VEGF Production by Flavonoids
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Abstract
Screening using a reporter under the control of the hypoxia-response element (HRE) identified several flavonoids and homoisoflavonoids that inhibit the activation of HRE under hypoxic conditions. Among various compounds, isorhamnetin, luteolin, quercetin, and methyl ophiopogonanone B (MOB) were effective at 3 to 9 μg/ml in inhibiting the reporter activity. The expression of vascular endothelial growth factor (VEGF) mRNA during hypoxia was also inhibited by MOB in HepG2 cells, but the effective doses were 10 to 20 μg/ml. MOB caused destabilization of hypoxia-inducible factor (HIF)-1α, as revealed by Western blotting, that was dependent on proteasome activity and the tumor suppressor, p53. The tubular formation and migration of human umbilical vein endothelial cells was also inhibited by MOB. MOB is expected to act as an inhibitor of angiogenesis.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 26 (10), 1379-1383, 2003
The Pharmaceutical Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390001204627651072
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- NII Article ID
- 110003608309
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- NII Book ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 6696326
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- PubMed
- 14519939
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed