- 【Updated on May 12, 2025】 Integration of CiNii Dissertations and CiNii Books into CiNii Research
- Trial version of CiNii Research Knowledge Graph Search feature is available on CiNii Labs
- 【Updated on June 30, 2025】Suspension and deletion of data provided by Nikkei BP
- Regarding the recording of “Research Data” and “Evidence Data”
Quantitative Analysis of Werner Helicase Activity Using the Single-Molecule Fluorescence Detection System MF10S
-
- Futami Kazunobu
- GeneCare (previously AGENE) Research Institute
-
- Goto Hideyuki
- GeneCare (previously AGENE) Research Institute
-
- Shimamoto Akira
- GeneCare (previously AGENE) Research Institute
-
- Watanabe Takahide
- GeneCare (previously AGENE) Research Institute
-
- Furuichi Yasuhiro
- GeneCare (previously AGENE) Research Institute
Search this article
Description
We developed a system that uses the single-molecule fluorescence detection system MF10S to assess quantitatively the activity of WRN helicase, the product of the causative gene of Werner syndrome that includes premature ageing. Double-strand DNA substrates labeled with the fluorescence dye TAMRA at the 5′ end and with a quencher at the 3′ end of the counter strand were incubated with a single trapper oligonucleotide and Werner helicase, and the resultant single DNA fragments labeled with TAMRA produced by the unwinding of WRN helicase were detected using the MF10S. The results using this system and those using polyacrylamide gel electrophoresis were well correlated. The MF10S system provides a quantitative analysis that is much faster, simpler, and more economical than systems using polyacrylamide gel electrophoresis and radioisotopes, and could be used as a quantitative analysis system for Werner helicase and other DNA helicase activities.
Journal
-
- Biological and Pharmaceutical Bulletin
-
Biological and Pharmaceutical Bulletin 28 (1), 9-12, 2005
The Pharmaceutical Society of Japan
- Tweet
Details 詳細情報について
-
- CRID
- 1390001204627755904
-
- NII Article ID
- 10016658372
-
- NII Book ID
- AA10885497
-
- DOI
- 10.1248/bpb.28.9
-
- ISSN
- 13475215
- 09186158
-
- NDL BIB ID
- 7200814
-
- PubMed
- 15635154
-
- Text Lang
- en
-
- Article Type
- journal article
-
- Data Source
-
- JaLC
- NDL Search
- Crossref
- PubMed
- CiNii Articles
- OpenAIRE
-
- Abstract License Flag
- Disallowed