Cloning and Characterization of a Gene Encoding Rac/Rop-Like Monomeric Guanosine 5'-Triphosphate-Binding Protein from Scoparia dulcis

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  • Mitamura Toshiaki
    Laboratory of Plant Resource Sciences, Graduate School of Medicine and Pharmaceutical Science for Research, University of Toyama
  • Shite Masato
    Laboratory of Plant Resource Sciences, Graduate School of Medicine and Pharmaceutical Science for Research, University of Toyama
  • Yamamura Yoshimi
    Laboratory of Plant Resource Sciences, Graduate School of Medicine and Pharmaceutical Science for Research, University of Toyama
  • Kurosaki Fumiya
    Laboratory of Plant Resource Sciences, Graduate School of Medicine and Pharmaceutical Science for Research, University of Toyama

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Abstract

A cDNA clone, designated Sd-racrop (969 bp), was isolated from seedlings of Scoparia dulcis. This gene contains an open reading frame encoding the protein of 197 amino acid residues with high homology to Rac/Rop small guanosine 5′-triphosphate-binding proteins from various plant sources. In Southern hybridization analysis, the restriction digests prepared from genomic DNA of S. dulcis showed a main signal together with a few weakly hybridized bands. The transcriptional level of Sd-racrop showed a transient decrease by exposure of the leaf tissues of S. dulcis to the ethylene-generating reagent 2-chloroethylphosphonic acid. However, an appreciable increase in gene expression was reproducibly observed upon treatment of the plant with methyl jasmonate. These results suggest that the Sd-racrop product plays roles in ethylene- and methyl jasmonate-induced responses of S. dulcis accompanying the change in the transcriptional level, however, the cellular events mediated by this protein toward these external stimuli would be regulated by various mechanisms.

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