Antioxidant Activities of Chitobiose and Chitotriose
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- Chen An-Shu
- Department of Pathobiochemistry, Faculty of Pharmacy, Meijo University
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- Taguchi Tadao
- Department of Pathobiochemistry, Faculty of Pharmacy, Meijo University
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- Sakai Kazuo
- Yaizu Suisankagaku Industry Co.
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- Kikuchi Kazuaki
- Yaizu Suisankagaku Industry Co.
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- Wang Min-Wei
- Department of Pharmacology, School of Pharmacy, Shenyang Pharmaceutical University
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- Miwa Ichitomo
- Department of Pathobiochemistry, Faculty of Pharmacy, Meijo University
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Abstract
Chitooligosaccharides, the oligomers made up of β-1,4-linked D-glucosamine, are obtained by partial hydrolysis of chitosan, a deacetylation product of chitin. The antioxidant activity of various chitooligosaccharides was tested in vitro with aminoguanidine, pyridoxamine, and Trolox as reference compounds. Hydroxylation of benzoate to salicylate by H2O2 in the presence of Cu2+ was effectively inhibited by chitobiose, chitotriose, aminoguanidine, pyridoxamine, and Trolox (their IC50 values=18, 80, 85, 10, and 95 μM, respectively), whereas glucosamine and N-acetylchito-oligosaccharides (di-N-acetylchitobiose and tri-N-acetylchitotriose) did not show any inhibitory activity. Chitobiose and chitotriose were more potent than the 3 reference compounds in scavenging hydroxyl radicals produced by photolysis of zinc oxide: IC50 values of the 2 oligomers were 30 and 55 μM, respectively. Such a scavenging activity of these 2 chitooligomers was also shown by the use of another system, a mixture of Fe3+/EDTA/ascorbate/H2O2, for producing hydroxyl radicals. Only chitobiose and Trolox, of the 10 compounds tested, had the ability to scavenge superoxide radicals generated by a non-enzymatic system using phenazine methosulfate and NADH. Taken together with our unpublished observation that chitobiose and chitotriose are appreciably absorbed from the intestine of rats, the present results suggest that these 2 chitooligosaccharides would act as effective antioxidants in vivo when orally ingested.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 26 (9), 1326-1330, 2003
The Pharmaceutical Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390001204628495744
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- NII Article ID
- 110003608596
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- NII Book ID
- AA10885497
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- COI
- 1:STN:280:DC%2BD3svislyjtw%3D%3D
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 6658268
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- PubMed
- 12951480
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed