Pharmacological Discrimination of Plasmalemmal and Mitochondrial Sodium–Calcium Exchanger in Cardiomyocyte-Derived H9c2 Cells
-
- Namekata Iyuki
- Department of Pharmacology, Toho University Faculty of Pharmaceutical Sciences
-
- Hamaguchi Shogo
- Department of Pharmacology, Toho University Faculty of Pharmaceutical Sciences
-
- Tanaka Hikaru
- Department of Pharmacology, Toho University Faculty of Pharmaceutical Sciences
この論文をさがす
抄録
We examined the effects of SEA0400 and CGP-37157 on the plasmalemmal Na+–Ca2+ exchanger (NCX) and mitochondrial NCX using H9c2 cardiomyocytes loaded with Ca2+-sensitive fluorescent probes. The plasmalemmal NCX activity, which was measured as the increase in cytoplasmic Ca2+ concentration after application of low Na+ extracellular solution, was inhibited by SEA0400 but not by CGP-37157. The mitochondrial NCX activity, which was measured in permeabilized H9c2 cells as the decrease in mitochondrial Ca2+ concentration after application of Ca2+-free extramitochondrial solution, was inhibited by CGP-37157 but not by SEA0400. These results indicate that SEA0400 and CGP-37157 act as selective inhibitors towards plasmalemmal and mitochondrial NCX, respectively, and provide pharmacological evidence that the plasmalemmal and mitochondrial NCX are distinct molecular entities.
収録刊行物
-
- Biological & Pharmaceutical Bulletin
-
Biological & Pharmaceutical Bulletin 38 (1), 147-150, 2015
公益社団法人 日本薬学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001204631233280
-
- NII論文ID
- 130004872234
-
- NII書誌ID
- AA10885497
-
- ISSN
- 13475215
- 09186158
-
- NDL書誌ID
- 026001230
-
- PubMed
- 25744471
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可