siRNA Delivery into Tumor Cells by Cationic Cholesterol Derivative-Based Nanoparticles and Liposomes
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- Hattori Yoshiyuki
- Institute of Medicinal Chemistry, Hoshi University
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- Hara Eriko
- Institute of Medicinal Chemistry, Hoshi University
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- Shingu Yumiko
- Institute of Medicinal Chemistry, Hoshi University
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- Minamiguchi Daiki
- Graduate School of Pharmaceutical Sciences, Kyoto University
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- Nakamura Ayako
- Institute of Medicinal Chemistry, Hoshi University
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- Arai Shohei
- Institute of Medicinal Chemistry, Hoshi University
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- Ohno Hiroaki
- Graduate School of Pharmaceutical Sciences, Kyoto University
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- Kawano Kumi
- Institute of Medicinal Chemistry, Hoshi University
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- Fujii Nobutaka
- Graduate School of Pharmaceutical Sciences, Kyoto University
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- Yonemochi Etsuo
- Institute of Medicinal Chemistry, Hoshi University
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説明
Previously, we reported that cationic nanoparticles (NP) composed of diamine-type cholesteryl-3-carboxamide (OH-Chol, N-(2-(2-hydroxyethylamino)ethyl)cholesteryl-3-carboxamide) and Tween 80 could deliver small interfering RNA (siRNA) with high transfection efficiency into tumor cells. In this study, we synthesized new diamine-type cationic cholesteryl carbamate (OH-C-Chol, cholesteryl (2-((2-hydroxyethyl)amino)ethyl)carbamate) and triamine-type carbamate (OH-NC-Chol, cholesteryl (2-((2-((2-hydroxyethyl)amino)ethyl)amino)ethyl)carbamate), and prepared cationic nanoparticles composed of OH-C-Chol or OH-NC-Chol with Tween 80 (NP-C and NP-NC, respectively), as well as cationic liposomes composed of OH-C-Chol or OH-NC-Chol with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) (LP-C and LP-NC, respectively) for evaluation of their possible use as siRNA delivery vectors. LP-C and LP-NC/siRNA complexes (lipoplexes) exhibited larger gene silencing effects than NP-C and NP-NC/siRNA complexes (nanoplexes), respectively, in human breast tumor MCF-7 cells, although the NP-C nanoplex showed high association with the cells. In particular, LP-NC lipoplex could induce strong gene suppression, even at a concentration of 5 nM siRNA. From these results, cationic liposomes composed of OH-NC-Chol and DOPE may have potential as gene vectors for siRNA transfection to tumor cells.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 38 (1), 30-38, 2015
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204631236736
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- NII論文ID
- 130004872237
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- NII書誌ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 026000788
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- PubMed
- 25744455
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- PubMed
- CiNii Articles
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- 使用不可