Antiasthmatic Effects of Resveratrol in Ovalbumin-Induced Asthma Model Mice Involved in the Upregulation of PTEN

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  • Chen Guo
    Department of Respiratory Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine
  • Tang JiHong
    Department of Respiratory Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine
  • Ni Zhenhua
    Central Lab, Putuo Hospital, Shanghai University of Traditional Chinese Medicine
  • Chen Qingge
    Department of Respiratory Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine
  • Li Zhao
    Department of Respiratory Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine
  • Yang Wei
    Department of Respiratory Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine
  • Din JianYun
    Department of Respiratory Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine
  • Luo Xumin
    Department of Respiratory Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine
  • Wang Xiongbiao
    Department of Respiratory Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine

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Resveratrol, a natural polyphenolic compound known for its antioxidative and antiinflammatory effects, exerts antiasthmatic effects, although the mechanism underlying these effects remains elusive. The phosphatase and tensin homology deleted on chromosome ten gene (PTEN) is involved in the pathogenesis of asthma, and PTEN overexpression in asthmatic mice improved asthma symptoms. To investigate whether the antiasthmatic mechanisms of resveratrol correlated with the upregulation of PTEN expression, an ovalbumin (OVA)-induced murine asthma model was used to determine the effectiveness of resveratrol treatment. PTEN mRNA and protein expression was assessed with real-time polymerase chain reaction (PCR) and immunochemistry. To determine whether airway remodeling occurred, the inner airway wall, mucous layer, and smooth muscle areas were each determined using an image analysis system. The lung epithelial cell line 16HBE was used to study the regulation of PTEN expression levels by resveratrol in vitro. Our data demonstrated that resveratrol inhibited OVA-induced airway inflammation and airway remodeling in asthmatic mice. PTEN expression was decreased in the murine asthma model, although the expression of PTEN was restored following treatment with resveratrol. Correlation efficiency analysis showed that PTEN expression was associated with the degree of airway remodeling. Further in vitro studies demonstrated that the inhibition of Sirtuin 1 (SIRT1) activity by a SIRT1 inhibitor and RNA interference decreased PTEN protein expression, while resveratrol attenuated the decreases in PTEN expression induced by the SIRT1 inhibitor. These data suggest the mechanism of the antiasthmatic effects of resveratrol in an OVA-induced murine asthma model, which resulted in the upregulation of PTEN via SIRT1 activation.

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