Cooperative Behavior of Fluoroquinolone Combinations against Escherichia coli and Staphylococcus aureus
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- Vitorino Graciela Pinto
- Departamento de Farmacia, Universidad Nacional de la Patagonia San Juan Bosco, Ciudad Universitaria
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- Becerra María Cecilia
- Departamento de Farmacia, IMBIV-CONICET. Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria
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- Barrera Gustavo Daniel
- Departamento de Química, Facultad de Ciencias Naturales, Universidad Nacional de la Patagonia San Juan Bosco, Ciudad Universitaria
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- Caira Mino Rodolfo
- Department of Chemistry, University of Cape Town
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- Mazzieri María Rosa
- Departamento de Farmacia, IMBIV-CONICET. Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria
書誌事項
- タイトル別名
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- Cooperative Behavior of Fluoroquinolone Combinations against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>
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説明
<p>The effects of different combinations of ciprofloxacin (CIP) and norfloxacin (NOR) against Escherichia coli and Staphylococcus aureus were studied using checkerboard, fractional inhibitory concentration (FIC) and time–kill analysis methods. Results obtained by the checkerboard method showed that the more effectives combinations against Escherichia coli were 0.0009 µg/mL CIP+0.0312 µg/mL NOR and 0.0037 µg/mL CIP+0.0075 µg/mL NOR with a FIC index of 0.62. For Staphylococcus aureus, the combination of 0.0625 µg/mL CIP+0.2500 µg/mL NOR showed a synergistic effect, with a FIC index of 0.50. The results of the time–kill method demonstrated either indifference or additivity of the combinations 0.0009 µg/mL CIP+0.0312 µg/mL NOR, 0.0018 µg/mL CIP+0.0312 µg/mL NOR, 0.0037 µg/mL CIP+0.0075 µg/mL NOR and 0.0037 µg/mL CIP+0.0156 µg/mL NOR at 24 h against E. coli. The combination 0.0037 µg/mL CIP+0.0312 µg/mL NOR showed synergistic activity. All the analyzed combinations evidenced bactericidal effects at 4 h. The combinations 0.0625 µg/mL CIP+0.2500 µg/mL NOR and 0.0625 µg/mL CIP+0.0625 µg/mL NOR showed indifference or additivity against S. aureus. None of them generated bactericidal effect at 4 h. Moreover, this last equimolecular combination (equivalent to 1/4 minimum inhibitory concentration (MIC) CIP+1/16 MIC NOR) generated higher reduction of nitro blue tetrazolium than drugs alone. By another way, combinations not equimolecular of CIP and NOR assayed, generated less levels of reactive oxygen species (ROS) than the components alone.</p>
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 40 (6), 758-764, 2017
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204632020224
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- NII論文ID
- 130005687991
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- NII書誌ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 028198139
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- PubMed
- 28566620
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可