Observation of Glycolytic Metabolites in Tumor Cell Lysate by Using Hyperpolarization of Deuterated Glucose
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- Kumagai Keiko
- Science Research Center, Kochi University
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- Akakabe Mai
- Science Research Center, Kochi University
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- Tsuda Masayuki
- Science Research Center, Kochi University
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- Tsuda Masashi
- Center for Advanced Marine Core Research, Kochi University
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- Fukushi Eri
- Research Faculty of Agriculture, Hokkaido University
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- Kawabata Jun
- Research Faculty of Agriculture, Hokkaido University
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- Abe Takamasa
- Oxford Instruments KK
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- Ichikawa Kazuhiro
- Innovation Center for Medical Redox Navigation, Kyushu University
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Hyperpolarization of stable isotope-labeled substrates and subsequent NMR measurement of the metabolic reactions allow for direct tracking of cellular reactions in vitro and in vivo. Here, we report the hyperpolarization of 13C6-glucose-d7 and evaluate its use as probes to observe glucose flux in cells. We measured the lifetime of the polarized signal governed by the spin–lattice relaxation time T1. 13C6-Glucose-d7 exhibited a T1 that was over ten times as long as that of 13C6-glucose, and metabolic NMR studies of hyperpolarized 13C6-glucose-d7 using tumor cell lysate led to observation of the resonances due to phosphorylated fluctofuranoses generated through aerobic glycolysis.
収録刊行物
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- Ｂｉｏｌｏｇｉｃａｌ ＆ Ｐｈａｒｍａｃｅｕｔｉｃａｌ Ｂｕｌｌｅｔｉｎ
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Ｂｉｏｌｏｇｉｃａｌ ＆ Ｐｈａｒｍａｃｅｕｔｉｃａｌ Ｂｕｌｌｅｔｉｎ 37 (8), 1416-1421, 2014
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204632210816
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- NII論文ID
- 130004677538
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- NII書誌ID
- AA10885497
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- COI
- 1:STN:280:DC%2BC2cbos1Khtg%3D%3D
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 025610325
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- PubMed
- 25087964
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可