Cellular Uptake of IgG Using Collagen-Like Cell-Penetrating Peptides

DOI Web Site Web Site PubMed 被引用文献2件 参考文献20件
  • Masuda Ryo
    Department of Chemistry and Biochemistry, School of Advanced Science and Engineering, Waseda University Research Institute for Science and Engineering, Waseda University
  • Yamamoto Kazuhiro
    Department of Chemistry and Biochemistry, School of Advanced Science and Engineering, Waseda University
  • Koide Takaki
    Department of Chemistry and Biochemistry, School of Advanced Science and Engineering, Waseda University

この論文をさがす

説明

Cell-penetrating peptides (CPPs) are attractive tools for delivering macromolecules that have poor membrane permeability, such as antibodies, into cells. However, the major drawback of conventional CPPs is their instability in bodily fluids. We previously reported a novel CPP employing a collagen-like triple-helical structure that exhibited remarkable resistance against serum proteases. Herein, we report the delivery of full-length immunoglobulin G (IgG) antibody into cells using a triple-helical CPP. The CPP was conjugated to IgG via a one-pot reaction using 2-iminothiolane as a crosslinking reagent. The triple-helical CPP was less prone to being aggregated and neutralized by serum than was octaarginine, a conventional CPP. However, most of the conjugates were found to be entrapped in endosomes.

収録刊行物

被引用文献 (2)*注記

もっと見る

参考文献 (20)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ