Effective mRNA Inhibition in PANC-1 Cells <i>in Vitro</i> Mediated <i>via</i> an mPEG–SeSe–PEI Delivery System

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  • Zhang Yuefeng
    Department of Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University
  • Yang Bin
    Department of Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University
  • Liu Yajie
    MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University
  • Qin Wenjie
    Department of Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University
  • Li Chao
    Department of Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University
  • Wang Lantian
    Department of Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University
  • Zheng Wen
    Department of Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University
  • Wu Yulian
    Department of Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University

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  • Effective mRNA Inhibition in PANC-1 Cells in Vitro Mediated via an mPEG–SeSe–PEI Delivery System

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Abstract

RNA interference (RNAi)-mediated gene therapy is a promising approach to cure various diseases. However, developing an effective, safe, specific RNAi delivery system remains a major challenge. In this study, a novel redox-responsive polyetherimide (PEI)-based nanovector, mPEG–SeSe–PEI, was developed and its efficacy evaluated. We prepared three mPEG–SeSe–PEI vector candidates for small interfering glyceraldehyde-3-phosphate dehydrogenase (siGADPH) and determined their physiochemical properties and transfection efficiency using flow cytometry and PEG11.6–SeSe–PEI polymer. We investigated the silencing efficacy of GADPH mRNA expression in PANC-1 cells and observed that PEG11.6–SeSe–PEI/siGADPH (N/P ratio=10) polyplexes possessed the appropriate size and zeta-potential and exhibited excellent in vitro gene silencing effects with the least cytotoxicity in PANC-1 cells. In conclusion, we present PEG11.6–SeSe–PEI as a potential therapeutic gene delivery system for small interfering RNA (siRNA).

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