Inhibitory Effect of Erythraline on Toll-Like Receptor Signaling Pathway in RAW264.7 Cells
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- Etoh Tadahiro
- Faculty of Pharmacy, Iwaki Meisei University
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- Kim Yong Pil
- Faculty of Pharmacy, Iwaki Meisei University
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- Ohsaki Ayumi
- Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University
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- Komiyama Kanki
- Kitasato Research Center of Environmental Science, Kitasato University
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- Hayashi Masahiko
- Faculty of Pharmacy, Iwaki Meisei University
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Erythraline, isolated from the bark of Erythrina crista-galli which are used as Brazilian medicine plant for the treatment of inflammation diseases, suppressed nitric oxide (NO) production and induction of inducible nitric oxide synthase (iNOS) expression in RAW264.7 cells stimulated by lipopolysaccharide (LPS). Because of Toll-like receptor (TLR) 4 and its signal transduction are indispensable to the production of NO and iNOS expression by LPS, we investigated the effects of erythraline on TLR signaling molecules. Western blot analysis revealed that the degradation of inhibitor of nuclear factor (NF)-κB (IκB) by LPS was suppressed by erythraline. Moreover, erythraline inhibited not only LPS-induced phosphorylation of IκB kinase (Ikk) but also phosphorylation of mitogen-activated protein kinases (MAPKs). However, it showed no effect on LPS -induced phosphorylation of transforming growth factor (TGF)-β-activated kinase (TAK) 1 that exists upstream of Ikk and MAPKs, and is required for the activation of these signaling molecules on TLR signaling pathway. These results suggested that erythraline might have inhibited the kinase activity of TAK1. Furthermore, these results were supported from the inhibitory pattern of erythraline on TLR signaling molecules when the cells were stimulated by TLR2 ligand, peptidoglycan which activates the same pathway as LPS on TLR signal transduction.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 36 (8), 1363-1369, 2013
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204633677568
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- NII論文ID
- 130003361488
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- NII書誌ID
- AA10885497
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- COI
- 1:STN:280:DC%2BC3sflslWluw%3D%3D
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 024716101
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- PubMed
- 23902980
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 使用不可