Ternary Complex of Plasmid DNA with Protamine and γ-Polyglutamic Acid for Biocompatible Gene Delivery System
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- Kanda Kosuke
- Department of Hospital Pharmacy, Nagasaki University Hospital
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- Kodama Yukinobu
- Department of Hospital Pharmacy, Nagasaki University Hospital
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- Kurosaki Tomoaki
- Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University
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- Imamura Masanobu
- Department of Hospital Pharmacy, Nagasaki University Hospital
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- Nakagawa Hiroo
- Department of Hospital Pharmacy, Nagasaki University Hospital
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- Muro Takahiro
- Department of Hospital Pharmacy, Nagasaki University Hospital
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- Higuchi Norihide
- Department of Hospital Pharmacy, Nagasaki University Hospital
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- Nakamura Tadahiro
- Department of Hospital Pharmacy, Nagasaki University Hospital
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- Kitahara Takashi
- Department of Hospital Pharmacy, Nagasaki University Hospital
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- Honda Masayuki
- Division of Medical Informatics, Nagasaki University Hospital
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- Sasaki Hitoshi
- Department of Hospital Pharmacy, Nagasaki University Hospital
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抄録
The purpose of the present study was to investigate the usefulness of the ternary complex with protamine and γ-polyglutamic acid (γ-PGA), which are biodegradable materials for foods and medical products, as a safe gene delivery vector. We formed cationic binary complexes (plasmid DNA (pDNA)/protamine complexes) with high transfection efficiency. The binary complex showed slight toxicity probably related to its total cationic charge. Then, we formed ternary complexes (pDNA/protamine/γ-PGA complexes) by addition of anionic polymer, γ-PGA, and they showed no cytotoxicity. The transfection efficiency of the pDNA/protamine/γ-PGA complexes was as high as that of the pDNA/protamine complexes, although their zeta potentials were different. Inhibition study of the gene expressions in B16-F10 cells suggested that pDNA/protamine complexes were taken up by caveolae-mediated endocytosis and macropinocytosis. On the other hand, pDNA/protamine/γ-PGA complexes were taken up by clathrin-mediated endocytosis and macropinocytosis. Thus, we succeeded in developing the ternary complex as a safe gene delivery vector with biocompatible materials.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 36 (11), 1794-1799, 2013
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204634317312
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- NII論文ID
- 130003382043
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- NII書誌ID
- AA10885497
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- COI
- 1:STN:280:DC%2BC2c7ivFymsg%3D%3D
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- ISSN
- 13475215
- 09186158
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- HANDLE
- 10069/34048
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- NDL書誌ID
- 024957796
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- PubMed
- 24189422
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
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