The Role of Fibronectin in Pulmonary Gene Transfer Following Intravenous Administration of Lipoplex in Mice
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- Yoshikawa Naoki
- Graduate School of Biomedical Sciences, Nagasaki University
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- Fumoto Shintaro
- Graduate School of Biomedical Sciences, Nagasaki University
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- Nakashima Mikiro
- Graduate School of Biomedical Sciences, Nagasaki University
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- Shimokawa Kenta
- Graduate School of Biomedical Sciences, Nagasaki University
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- Miyamoto Hirotaka
- Graduate School of Biomedical Sciences, Nagasaki University
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- Nishida Koyo
- Graduate School of Biomedical Sciences, Nagasaki University
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We analyzed the effect of serum and fibronectin on pulmonary transgene expression after intravenous injection of cationic liposome–plasmid DNA (pDNA) complex (lipoplex) in mice. 1,2-Dioleoyl-3-trimethylammonium-propane (DOTAP) methyl sulfate salt/cholesterol lipoplex was incubated with several serum components for 5 min at 37°C prior to injection. We analyzed pulmonary transgene expression and pulmonary accumulation of lipoplex. While interaction with serum did not decrease pulmonary transgene expression, interaction with heat-inactivated serum did decrease it. Moreover, interaction with fibronectin enhanced pulmonary transgene expression. Inhibition of the binding of fibronectin to integrin decreased pulmonary transgene expression after injection of untreated lipoplex. We found that pulmonary accumulation of lipoplex changed depending on the kind of interacting serum components after injection. Furthermore, interaction with fibronectin increased pulmonary accumulation of lipoplex. Interaction with serum was required for pulmonary gene transfer following intravenous injection of lipoplex. Fibronectin appears to be a particularly critical component. Furthermore, the binding of fibronectin interacting with lipoplex to integrin was an important mechanism for pulmonary transgene expression.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 36 (11), 1807-1813, 2013
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204634320384
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- NII論文ID
- 130003382045
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- NII書誌ID
- AA10885497
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- COI
- 1:STN:280:DC%2BC2c7ivFymsA%3D%3D
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- ISSN
- 13475215
- 09186158
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- HANDLE
- 10069/34049
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- NDL書誌ID
- 024957821
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- PubMed
- 24189424
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 使用不可