Efficient Intradermal Delivery of Superoxide Dismutase Using a Combination of Liposomes and Iontophoresis for Protection against UV-Induced Skin Damage
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- Kigasawa Kaoru
- Graduate School of Life Science, Hokkaido University
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- Miyashita Moeko
- Graduate School of Life Science, Hokkaido University
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- Kajimoto Kazuaki
- Faculty of Pharmaceutical Sciences, Hokkaido University
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- Kanamura Kiyoshi
- TTI Ellebeau Inc.
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- Harashima Hideyoshi
- Faculty of Pharmaceutical Sciences, Hokkaido University
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- Kogure Kentaro
- Department of Biophysical Chemistry, Kyoto Pharmaceutical University
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抄録
Superoxide dismutase (SOD) is a potent antioxidant agent that protects against UV-induced skin damage. However, its high molecular weight is a significant obstacle for efficient delivery into the skin through the stratum corneum and development of antioxidant activity. Recently, we developed a non-invasive transfollicular delivery system for macromolecules using a combination of liposomes and iontophoresis, that represents promising technology for enhancing transdermal administration of charged drugs (IJP, 403, 2011, Kajimoto et al.). In this study, in rats we attempted to apply this system to intradermal delivery of SOD for preventing UV-induced skin injury. SOD encapsulating in cationic liposomes was subjected to anodal iontophoresis. After iontophoretic treatment, the liposomes were diffused widely in the viable skin layer around hair follicles. In contrast, passive diffusion failed to transport liposomes efficiently into the skin. Iontophoretic delivery of liposomes encapsulating SOD caused a marked decrease in the production of oxidative products, such as malondialdehyde, hexanoyl lysine, and 8-hydroxi-2-deoxyguanosine, in UV-irradiated skin. These findings suggested that functional SOD can be delivered into the skin using a combination of iontophoresis and a liposomal system. In conclusion, we succeeded in developing an efficient intradermal SOD delivery system, that would be useful for delivery of other macromolecules.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 35 (5), 781-785, 2012
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204634465408
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- NII論文ID
- 130001872240
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- NII書誌ID
- AA10885497
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- COI
- 1:STN:280:DC%2BC38jgslelsw%3D%3D
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 023593063
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- PubMed
- 22687417
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可