Reliability and clinical utility of Enzyme-linked immunosorbent assay for detection of anti-aminoacyl-tRNA synthetase antibody
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- ABE Takeo
- Division of Rheumatology, Department of Internal Medicine, Hyogo College of Medicine
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- TSUNODA Shinichiro
- Division of Rheumatology, Department of Internal Medicine, Hyogo College of Medicine
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- NISHIOKA Aki
- Division of Rheumatology, Department of Internal Medicine, Hyogo College of Medicine
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- AZUMA Kouta
- Division of Rheumatology, Department of Internal Medicine, Hyogo College of Medicine
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- TSUBOI Kazuyuki
- Division of Rheumatology, Department of Internal Medicine, Hyogo College of Medicine
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- OGITA Chie
- Division of Rheumatology, Department of Internal Medicine, Hyogo College of Medicine
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- YOKOYAMA Yuichi
- Division of Rheumatology, Department of Internal Medicine, Hyogo College of Medicine
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- FURUKAWA Tetsuya
- Division of Rheumatology, Department of Internal Medicine, Hyogo College of Medicine
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- MARUOKA Momo
- Division of Rheumatology, Department of Internal Medicine, Hyogo College of Medicine
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- TAMURA Masao
- Division of Rheumatology, Department of Internal Medicine, Hyogo College of Medicine
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- YOSHIKAWA Takahiro
- Division of Rheumatology, Department of Internal Medicine, Hyogo College of Medicine
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- SAITO Atsushi
- Division of Rheumatology, Department of Internal Medicine, Hyogo College of Medicine
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- SEKIGUCHI Masahiro
- Division of Rheumatology, Department of Internal Medicine, Hyogo College of Medicine
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- AZUMA Naoto
- Division of Rheumatology, Department of Internal Medicine, Hyogo College of Medicine
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- KITANO Masayasu
- Division of Rheumatology, Department of Internal Medicine, Hyogo College of Medicine
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- MATSUI Kiyoshi
- Division of Rheumatology, Department of Internal Medicine, Hyogo College of Medicine
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- HOSONO Yuji
- Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University
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- NAKASHIMA Ran
- Department of Rheumatology and Clinical Immunology, Kyoto University Hospital
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- OHMURA Koichiro
- Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University
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- MIMORI Tsuneyo
- Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University
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- SANO Hajime
- Division of Rheumatology, Department of Internal Medicine, Hyogo College of Medicine
Bibliographic Information
- Other Title
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- 抗ARS抗体ELISAキットの信頼性と臨床応用についての検討
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Abstract
Anti-aminoacyl-tRNA synthetase (ARS) antibody is one of the myositis-specific autoantibodies to make a diagnosis of polymyositis (PM) and dermatomyositis (DM). Recently a new enzyme-linked immunosorbent assay (ELISA) kit of concurrently detected anti-ARS antibodies (anti-Jo-1, anti-PL-7, anti-PL-12, anti-EJ and anti-KS) have become to measure in the clinical setting. To evaluate the reliability of this ELISA kit, we measured anti-ARS antibodies in 75 PM and DM patients using by this ELISA assay and compared them with the results by RNA immunoprecipitation assay. Between the measurements of anti-PL-7, anti-PL-12, anti-EJ and anti-KS autoantibodies by ELISA assay and RNA-IP assay, the concordance rate of reproducibility is 95.1% and the positive agreement rate is 90.9% and negative agreement rate is 96.0% and kappa statistic is 0.841. Between the measurements of existing anti-Jo-1 antibody ELISA kit and anti-ARS antibody ELISA kit, the concordance rate of reproducibility is 96.9%, the positive agreement rate is 100%, negative agreement rate is 96.1% and kappa statistic is 0.909. The lung involvement in patients with PM and DM patients are positive of anti-ARS antibodies and anti-melanoma differentiation associated gene5 (MDA5) antibody at a rate around 70%. Then most life-threatening ILD with anti-MDA5 positive clinically amyopathic dermatomyositis patients could be highly guessed when anti-ARS antibodies are negative.
Journal
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- Japanese Journal of Clinical Immunology
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Japanese Journal of Clinical Immunology 39 (2), 140-144, 2016
The Japan Society for Clinical Immunology