Simultaneous and Selective Determination of Nitrite, Nitrate and Phosphate Ions in Seawater by Ion Chromatography

DOI Web Site Web Site
  • HORIOKA Yuuta
    Cluster of Biotechnology and Chemistry Systems, Graduate School of Systems Engineering, Kinki University
  • KURATA Shoichi
    Department of Biotechnology and Chemistry, Faculty of Engineering, Kinki University
  • ITO Kazuaki
    Cluster of Biotechnology and Chemistry Systems, Graduate School of Systems Engineering, Kinki University Department of Biotechnology and Chemistry, Faculty of Engineering, Kinki University

Bibliographic Information

Other Title
  • イオンクロマトグラフィーによる海水中の亜硝酸イオン，硝酸イオン及びリン酸イオンの選択的同時定量
  • イオンクロマトグラフィー ニ ヨル カイスイチュウ ノ アショウサン イオン,ショウサン イオン オヨビ リンサン イオン ノ センタクテキ ドウジ テイリョウ

Search this article

Abstract

The simultaneous determination of nitrite (NO2−), nitrate (NO3−), and phosphate (PO43−) in seawater samples was examined by ion chromatography. Nitrite and nitrate ions were separated on dilauryldimethylammonium (DDA+)-coated monolithic ODS columns using 0.4 M sodium chloride (pH = 5.0) as the eluent, and were detected by UV at 225 nm without any interferences by the major anions in seawater. Phosphate ion was detected at 885 nm by a post-column reaction based on the formation of Molybdenum Blue using sulfuric acid, hexaammonium heptamolybdate tetrahydrate, antimony potassium tartrate, L(+)-ascorbic acid, and sodium dodecyl sulfate. Thus, this method enabled the direct determination of nitrite, nitrate, and phosphate ions in seawater. Calibration curves of all ions in 35‰ artificial seawater by a peak-area method showed good linearity (R2≥0.998) in the range of 0 – 2 mg L−1 for NO2− and NO3−, and 0 – 2 mg-P L−1 (as phosphorous) for PO43−. The detection limits (S/N = 3) of nitrite, nitrate, and phosphate were 0.8 μg L−1, 1.6 μg L−1, and 0.09 μg-P L−1, respectively. Also, the repeatability (1 mg L−1 for NO2− and NO3−, and 1 mg-P L−1 for PO43−, n = 5) was below 0.05% (retention time), 0.66% (peak area), and 0.69% (peak height). The present method was applied to the simultaneous and selective determination of analytes in seawater samples of Seto Inland Sea. The recovery rate by the analysis of spiked samples was in the rage of 93 – 105%.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 63 (8), 657-663, 2014

    The Japan Society for Analytical Chemistry

Keywords

Details 詳細情報について

Report a problem

Back to top