Spectrophotometric Determination of Manganese(II) and Basic Polyamino Acids with o-Sulfophenylfluorone

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  • KAJI Shinya
    Osaka University of Pharmaceutical Sciences
  • TOMIDA Mayu
    Osaka University of Pharmaceutical Sciences
  • HOSHINO Mitsuru
    Osaka University of Pharmaceutical Sciences
  • KAMINO Shinichiro
    Laboratory of Multiple Molecular Imaging Research Center for Molecular Imaging Science RIKEN Kobe Institute Department of Pharmaceutical Analytical Chemistry Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
  • ASANO Mamiko
    Osaka University of Pharmaceutical Sciences
  • YAMAGUCHI Takako
    Osaka University of Pharmaceutical Sciences
  • FUJITA Yoshikazu
    Osaka University of Pharmaceutical Sciences

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Other Title
  • ｏ－スルホフェニルフルオロンを用いるマンガン（ＩＩ）及び塩基性ポリアミノ酸の吸光光度定量
  • o-スルホフェニルフルオロンを用いるマンガン(2)及び塩基性ポリアミノ酸の吸光光度定量
  • o スルホフェニルフルオロン オ モチイル マンガン 2 オヨビ エンキセイ ポリ アミノサン ノ キュウコウ コウド テイリョウ

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Abstract

A spectrophotometric method was established for the determination of manganese(II) {Mn(II)} and Polylysine (PolyL) with o-sulfophenylfluorone (SPF). Beer’s law was obeyed in the range of 0.06 ∼ 3.3 μg for Mn(II) in a basic medium with an apparent molar absorptivity(ε) at 565 nm, the relative standard deviation (RSD) being 1.63 × 105 L mol−1 cm−1 and 1.46% (</i>n</i> = 5). This method is about 2-70 times more sensitive than the existing spectrophptpmetric methods, and can determine more easily. Also, the method was successfully applied to assays of Mn(II) in real samples. The thermodynamic parameters suggest that the colored complex formed in this reaction system is an association complex between [Mn(II) – SPF] and [TSAC]. Next, Beer’s law was obeyed in the range of 1.0 ∼ 60 μg for PolyL, a basic solution. The ε and RSD are 4.18 × 106 L mol−1 cm−1 (molar weight ≈ 225000) at 565 nm and 1.49% (n = 5), respectively. This method can determine polymers, not monomers. Furthermore, this method was applied to recovery tests of PolyL in real samples ; the results were satisfactory.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 60 (8), 619-627, 2011

    The Japan Society for Analytical Chemistry

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