Spectrophotometric Determination of Manganese(II) and Basic Polyamino Acids with o-Sulfophenylfluorone

KAJI Shinya, TOMIDA Mayu, HOSHINO Mitsuru, KAMINO Shinichiro, ASANO Mamiko, YAMAGUCHI Takako, FUJITA Yoshikazu

doi:10.2116/bunsekikagaku.60.619

Bibliographic Information

Other Title

ｏ－スルホフェニルフルオロンを用いるマンガン（ＩＩ）及び塩基性ポリアミノ酸の吸光光度定量
o-スルホフェニルフルオロンを用いるマンガン(2)及び塩基性ポリアミノ酸の吸光光度定量
o スルホフェニルフルオロンオモチイルマンガン 2 オヨビエンキセイポリアミノサンノキュウコウコウドテイリョウ

Search this article

Abstract

A spectrophotometric method was established for the determination of manganese(II) {Mn(II)} and Polylysine (PolyL) with o-sulfophenylfluorone (SPF). Beer’s law was obeyed in the range of 0.06 ∼ 3.3 μg for Mn(II) in a basic medium with an apparent molar absorptivity(ε) at 565 nm, the relative standard deviation (RSD) being 1.63 × 10⁵ L mol⁻¹ cm⁻¹ and 1.46% (</i>n</i> = 5). This method is about 2-70 times more sensitive than the existing spectrophptpmetric methods, and can determine more easily. Also, the method was successfully applied to assays of Mn(II) in real samples. The thermodynamic parameters suggest that the colored complex formed in this reaction system is an association complex between [Mn(II) – SPF] and [TSAC]. Next, Beer’s law was obeyed in the range of 1.0 ∼ 60 μg for PolyL, a basic solution. The ε and RSD are 4.18 × 10⁶ L mol⁻¹ cm⁻¹ (molar weight ≈ 225000) at 565 nm and 1.49% (n = 5), respectively. This method can determine polymers, not monomers. Furthermore, this method was applied to recovery tests of PolyL in real samples ; the results were satisfactory.

Journal

BUNSEKI KAGAKU

BUNSEKI KAGAKU 60 (8), 619-627, 2011

The Japan Society for Analytical Chemistry

Keywords

Details 詳細情報について

Export