ペンタフルオロフェニル構造を用いる分離指向性誘導体化−HPLC分析法の開発

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タイトル別名
  • Development of the Separation-oriented Derivatization Method in HPLC Analysis Using Pentafluorophenyl Moiety
  • ペンタフルオロフェニル構造を用いる分離指向性誘導体化-HPLC分析法の開発
  • ペンタフルオロフェニル コウゾウ オ モチイル ブンリ シコウセイ ユウドウタイカ-HPLC ブンセキホウ ノ カイハツ

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抄録

We have developed a new separation-oriented derivatization method based on the affinity of the interaction between the pentafluorophenyl (PFP) group for the HPLC determination of native fluorescent non-steroidal anti-inflammatory drugs (NSAIDs). This method involves the derivatization of NSAIDs with pentafluorobenzyl bromide and the separation of derivatives using a PFP-modified stationary phase column. In this method, the PFP-derivatized NSAIDs were selectively retained on the PFP column, followed by fluorescence detection, whereas the derivatives were not retained on the ODS-, phenyl-, and perfluoroalkyl-modified HPLC columns. The detection limits (signal-to-noise ratio of 3) of the NSAID standards were found to be 44 – 84 fmol on column for a 20 μL injection, and the repeatability of this method was within 7.1% as relative standard deviation. We show that the proposed method can be used to analyze NSAIDs in human plasma samples without the interference of endogenous matrices.

収録刊行物

  • 分析化学

    分析化学 62 (8), 713-717, 2013

    公益社団法人 日本分析化学会

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