Evaluation of Cryosurgery-Hyperthermia Treatment Utilizing Peltier Thermoelectric Effect for Living Tissue

DOI

Bibliographic Information

Other Title
  • ペルチェ熱電効果を利用した凍結温熱手術の生体組織に対する評価

Abstract

Cryosurgery and hyperthermia treatment are used as a treatment method for malignant tumors. Since liquid nitrogen is used as the cryogens, it is difficult to control the freezing rate and thawing rate. In hyperthermia, there are problems of thermotolerance acquisition by heat shock protein (HSP) and only a few studies regarding hyperthermia with cryosurgery have been investigated. The aims of this study are to produce cryosurgery-hyperthermia system utilizing Stirling Cooler and Peltier device and evaluate hyperthermia after cryosurgery by comparing cryosurgery and hyperthermia on the mouse liver. Normal living liver tissues of mice are divided into 3 groups (cryosurgery and cryosurgery-hyperthermia, hyperthermia), then performed cryosurgery, hyperthermia and hyperthermia followed cryosurgery, applying a 1 cycle rapid freezing and slow thawing method for cryosurgery. The temperatures of the tissue surface and probe were measured during operation, the liver was stained by Hematoxylin-Eosin (HE) after operation and observed under an optical microscope. The results showed measured temperature of rapid freezing and slow thawing. HE stained tissue showed stasis, pyknosis, nucleus disappearance and decreasing stainability in cryosurgery and cryosurgery-hyperthermia group, stasis, pyknosis and degreasing stainability in hyperthermia group. The results suggested cryosurgery-hyperthermia was the most effective to destroy the tissue.

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Details 詳細情報について

  • CRID
    1390001204667019520
  • NII Article ID
    130004697553
  • DOI
    10.11322/tjsrae.24.183
  • COI
    1:CAS:528:DC%2BD2sXhsVSku7bO
  • ISSN
    2185789X
    13444905
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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