書誌事項
- タイトル別名
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- <I>In situ</I> RT-PCR: Identification of specific cells using a high sensitive detection of mRNA
- 高感度mRNA検出法で細胞を同定する
- コウカンド mRNA ケンシュツホウ デ サイボウ オ ドウテイスル
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Recently, in situ reverse transcription (RT)-PCR has been developed to detect weak mRNA signals on histological sections by amplifying the relevant molecular mRNA, because RT-PCR allows one mRNA to be amplified a billionfold. This method was performed using rTth DNA polymerase that transcripts cDNA to mRNA, and amplifying the cDNA. We applied this technique to detect prohormone convertases PC1 and PC2 in the rat pituitary gland, and vascular endothelial growth factor (VEGF) in the developing pituitary gland, where these mRNAs were not detectable usual in situ hybridization techniques. Therefore, this in situ RT-PCR method is useful for identifying specific cells expressing weak signals in histological sections.
収録刊行物
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- 比較生理生化学
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比較生理生化学 24 (3), 126-129, 2007
日本比較生理生化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204668686976
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- NII論文ID
- 10025859220
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- NII書誌ID
- AN10391932
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- COI
- 1:CAS:528:DC%2BD2sXhtFGht7bP
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- ISSN
- 18819346
- 09163786
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- NDL書誌ID
- 8917396
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
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- 抄録ライセンスフラグ
- 使用不可