[Updated on Apr. 18] Integration of CiNii Articles into CiNii Research

Relationship between the cAMP levels in leukocytes and the cytokine balance in patients surviving gram negative bacterial pneumonia

  • Matsumoto Tomio
    Department of Surgery, Division of Frontier Medical Science, Graduate School of Biomedical Sciences, Hiroshima University
  • Hayamizu Keisuke
    Major in Medical Engineering and Technology, Graduate School of Medical Technology and Health Welfare Sciences, Hiroshima International University
  • Marubayashi Seiji
    Department of Surgery, Ajina Tsuchiya Hospital
  • Shimizu Kiyoshi
    Major in Medical Engineering and Technology, Graduate School of Medical Technology and Health Welfare Sciences, Hiroshima International University
  • Hamamoto Aki
    Major in Medical Engineering and Technology, Graduate School of Medical Technology and Health Welfare Sciences, Hiroshima International University
  • Yamaguchi Tsuyoshi
    Department of Surgery, Division of Frontier Medical Science, Graduate School of Biomedical Sciences, Hiroshima University
  • Hashizume Junji
    Department of Surgery, Division of Frontier Medical Science, Graduate School of Biomedical Sciences, Hiroshima University
  • Onabe Takashi
    Department of Surgery, Onabe Surgical Hospital
  • Asahara Toshimasa
    Department of Surgery, Division of Frontier Medical Science, Graduate School of Biomedical Sciences, Hiroshima University
  • Ohdan Hideki
    Department of Surgery, Division of Frontier Medical Science, Graduate School of Biomedical Sciences, Hiroshima University

Search this article

Abstract

Lipopolysaccharide-stimulated leukocytes secrete proinflammatory cytokines including tumor necrosis factor-α and interleukin-12. Over-activation of host defense systems may result in severe tissue damage and requires regulation. Granulocyte colony-stimulating factor and interleukin-10 are candidate cytokines for inducing tolerance to lipopolysaccharide re-stimulation. We compared cytokines secreted by lipopolysaccharide-stimulated blood cells from patients who had survived gram negative bacterial pneumonia (Pseudomonas aeruginosa, Escherichia coli or Proteus mirabilis, n = 26) and age-matched healthy volunteers (n = 18). Interleukin-12p70 and tumor necrosis factor-α expression was significantly lower in patients (p = 0.0039 and p<0.001) compared to healthy controls, while granulocyte colony-stimulating factor production was markedly higher in patients (p<0.001). Levels of interleukin-10 were comparable. Granulocyte colony-stimulating factor expression was inversely correlated with interleukin-12p70 (R = −0.71, p<0.001) and tumor necrosis factor-α (R = −0.64, p<0.001) expression; interleukin-10 showed no significant correlation. In unstimulated leukocytes from patients, cAMP levels were significantly raised (p = 0.020) and were correlated inversely with interleukin-12p70 levels (R = −0.81, p<0.001) and directly with granulocyte colony-stimulating factor (R = 0.72, p = 0.0020), matrix metalloproteinase-9 (R = 0.67, p = 0.0067) and interleukin-10 (R = 0.54, p = 0.039) levels. Our results demonstrate that granulocyte colony-stimulating factor production by lipopolysaccharide-stimulated leukocytes is a useful indicator of tolerance induction in surviving pneumonia patients and that measuring cAMP in freshly isolated leukocytes may also be clinically significant.<br>

Journal

References(41)*help

See more

Details

Report a problem

Back to top