Differentiation and Monitoring of Cells Using a Biochip for Regenerative Medicine : Differentiation of Bone Marrow Stromal Cells and Myoblasts
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- UCHIDA Tomoyuki
- Department of Micro-Nano Systems Engineering, Nagoya University
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- ARAI Fumihito
- Department of Bioengineering and Robotics, Tohoku University
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- SUZUKI Osamu
- Department of Dentistry, Tohoku University
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- ICHIKAWA Akihiko
- National Institute of Advanced Industrial Science and Technology, Intelligent Systems Research Institute, Ubiquitous Functions Research Group
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- FUKUDA Toshio
- Department of Micro-Nano Systems Engineering, Nagoya University
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- KATAGIRI Takenobu
- Department of Dentistry, Saitama Medical School
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- KAMIJO Ryutaro
- Department of Dentistry, Showa University
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- NAKAMURA Masanori
- Department of Dentistry, Showa University
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- NUMATA Mamoru
- JGC Corporation
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- WATANABE Naruaki
- JGC Corporation
書誌事項
- タイトル別名
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- Differentiation and Monitoring of Cells Using a Biochip for Regenerative Medicine
- (Differentiation of Bone Marrow Stromal Cells and Myoblasts)
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抄録
A novel biochip is developed for culturing stem cells. Biochip is made of Polymer (PDMS), and cells can be loaded by gradient strains in one chip. They grow well on a hydrophilic membrane and differentiation is promoted by cyclic strains. In this paper, we propose the method for culturing and monitoring of stem cells such as bone marrow stromal cells (ST2 cells) and myoblasts (C2C12 cells), and the results of culture. First we analyzed strains on a membrane when an air hole is decompressed, and clarified their range. From experiment, bone marrow stromal cells grew well in a narrow range, and we quantified their ALP activity as a measure of differentiation. As myoblasts, the direction of their differentiation was perpendicular to a groove, that is, the same direction of uniaxial strains.
収録刊行物
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- JSME International Journal Series C Mechanical Systems, Machine Elements and Manufacturing
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JSME International Journal Series C Mechanical Systems, Machine Elements and Manufacturing 49 (3), 852-858, 2006
一般社団法人 日本機械学会
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詳細情報 詳細情報について
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- CRID
- 1390001204677834112
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- NII論文ID
- 110004781785
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- NII書誌ID
- AA11179487
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- ISSN
- 1347538X
- 13447653
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- HANDLE
- 10097/50894
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- NDL書誌ID
- 8048868
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
