Regulation of LAK Activity from Pleural Cavity Lymphocytes and Peripheral Blood Lymphocytes by Pleural Cavity Macrophages in Lung Cancer Patients without Malignant Effusion.

Takahashi Keiji, Sone Saburo, Uyama Tadashi, Sumitomo Masayuki, Saito Seiya, Ogura Takeshi, Monden Yasumasa

doi:10.2482/haigan.34.371

The ability of pleural cavity lymphocytes (PLY) and peripheral blood lymphocytes (BLY) to generate lymphokine (IL-2)-activated killer (LAK) activity and of pleural cavity macrophages (PCM) and peripheral blood monocytes (Mo) to regulate LAK induction were examined. Purified PLY, BLY (>99%) and PCM, Mo (>90%) were separated by two-step discontinuous gradient centrifugation. LAK activity against Daudi cells was measured by 4-hr 51Cr release assay. Results showed that non-stimulated PCM and LPS-stimulated PCM could up-regulate LAK activity from BLY significantly (p<0.05, p<0.05). LAK activity induced from PLY could be up-regulated significantly by only LPS-stimulated PCM (p<0.05). LAK activity induced from BLY could be up-regulated significantly by only non-stimulated Mo (p<0.01). Non-stimulated Mo up-regulated LAK activity induced from BLY significantly more than LPS-stimulated Mo (p<0.05). These findings indicate that the function of PCM to regulate LAK activity from BLY and PLY is different from those of Mo and that PCM is the most effective cell against the invasion of cancer cells into the pleural cavity of lung cancer patients.

Regulation of LAK Activity from Pleural Cavity Lymphocytes and Peripheral Blood Lymphocytes by Pleural Cavity Macrophages in Lung Cancer Patients without Malignant Effusion.

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