Microdissection and micromanipulation in the scanning electron microscope.
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- Wakita Minoru
- Department of Oral Anatomy II, Hokkaido University School of Dentistry
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- Shioi Takashi
- Department of Oral Anatomy II, Hokkaido University School of Dentistry
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- Nodasaka Yoshinobu
- Electron Microscope Laoratory, Hokkaido University School of Dentistry
Abstract
Microdissection under SEM was attempted by using a micromanipulator functioning with screws and equipped with a φ 0.1mm tungsten wire as the tip instrument. The specimens in these experiments, were rat embryos immersed in a 1.25% glutaraldehyde (GA)-2% paraformaldehyde (PFA) mixture and some organs from adult rats perfused with 2.5% GA-4% PFA mixture. It was shown that the Bowman's capsule of the kidney corpuscle exposed on the freely fractured surface can be removed by a tungsten probe on the micromanipulator, and to make the gromerulus visible, the pericardium of the rat embryo could be peeled off and the anlage of the heart exposed and dissected further to display the intracardial space. Microdissection of the eye of an embryo was also tried.<BR>It showed the possiblility of freely and arbitrarily making objects visible, objects which until now have been obtained only by chance. This method will have wider applications especially in embryological studies, which depend on the skill of experienced investigators.<BR>The various problems to develop more accurate microdissections were pointed out and discussed. The solutions to some of these problems are suggested.
Journal
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- Japanese Journal of Oral Biology
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Japanese Journal of Oral Biology 26 (2), 502-517, 1984
Japanese Association for Oral Biology
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Details 詳細情報について
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- CRID
- 1390001204681044352
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- NII Article ID
- 130003815070
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- ISSN
- 03850137
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed