ヒト歯髄培養細胞におけるStreptococcus mutansによる細胞死の誘導

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  • Study on Apoptosis of the Human Dental Pulp Cell induced by S. mutans

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Streptococcus mutans (S. mutans) has been implicated as a primary causative agent of dental caries in humans. S. mutans may bind on to dental pulp cells, there by inducing progressive caries. In this study the author investigated how S. mutans is related to the cell death of human dental pulp. The author of the present study furthermore examined whether or not S. mutans causes apoptosis.<BR>Cells were cultured for 48 hr using serum-free medium with S. mutans. Cytotoxicity was tested on the cells with the MTT assay, the LDH assay and the BrdU assay. The induction of DNA fragmentation of the cells was examined with ELISA, agarose electrophoresis, and flow cytometric analysis. Cell death induced by S. mutans occurred via apoptosis, increased fragmented DNA, and the typical ladder pattern of DNA fragmentation indicative of apoptosis.<BR>These findings suggest that S. mutans causes apoptosis in pulp cells, indicating that S. mutans contributes to the initiation of infection of pulp in vivo.

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