ヒト培養歯根膜細胞におけるＰｒｏｔｅｏｇｌｙｃａｎ産生に及ぼすＴｒａｎｓｆｏｒｍｉｎｇ　Ｇｒｏｗｔｈ　Ｆａｃｔｏｒ‐β（ＴＧＦ‐β）の影響について

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  • Stimulation of Proteoglycan by Transforming Growth Factor-.BETA. on human periodontal ligament fibroblast.

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Transforming growth factor-β(TGF-β) is a multifunctional polypeptide th at influences proliferation and differentiation in various types of cell. It has been recognized that platelets and bone are rich sources of TGF-β. However, using molecular hybridization, it has been demonstrated that periodontal cells also expressed a high level of TGF-β mRNA, relative to bone cells. This studyh as been extended on human periodontal ligament fibroblast (HPLF) by investig ating the effect of TGF-β on the synthesis of proteoglycans, which are believed to be an important factor in bone remodeling. Cells were incubated to be labeled with 35S-sulfate. The expression of proteoglycan mRNA was also observed by northern blots. Utilizing the characterized data of HPLF cells (Sommerman, M. J. et al., J. Dent. Res., 67: 66, 1988), the cells which had high alkaline phosphatase (ALP) activity were chosen. On HPLF cell-line produced predominantly the proteogl ycan II (Decorin; PG II). No stimulating effects of PG II synthesis were observed by TGF -β. However, TGF-β stimulated the expression of mRNA of proteoglycan I (Biglycan; PG I) and the synthesis of 35S-sulfate labeled PG I. According to the time course study, mRNA of PG I was increased after 12 h exposure to TGF-β. These results suggest that the PG I synthesis might be enhanced by indirect TGF-β effects and PG I synthesis is believed to be important for periodontal remodeling.

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