スナネズミ味細胞の甘味応答を誘発すると考えられる２つのセカンドメッセンジャー

DOI

書誌事項

タイトル別名
  • Dual pathways for sweet taste transduction in isolated gerbil taste cells: cAMP and IP3 mediated mechanisms.

抄録

Mechanisms of sweet taste transduction in gerbil taste cells were examined using the conventional whole-cell patch-clamp technique. Outward K+ currents of the taste cell induced by depolarizing electric pulses were reduced by 10 mM Na-saccharin, but were enhanced by amino acid sweeteners of 10 mM D-tryptophan. The outward K+ current was also enhanced by external application of Ca2+ -ionophore, 5μM ionomycin and intracelluar application of 5μM IP3, (inositol-1, 4, 5-trisphosphate). These results suggest that the sweet taste transduction mechanism for Na-saccharin is concerned with an increase of the intracellular cAMP level, while that for D-tryptophan is concerned with an increase of the intracellular IP3, level causing Ca2+ release from the internal stores. Gurmarin, an inhibitor of sweet taste. antagonized the suppressive effect of Na-saccharin on outward K+ currents, but did not affect the enhancing effect of Dtryptophan on outward K+ currents. The results from treatment with gurmarin suggest that the receptor site for Na-saccharin is different from that for D-tryptophan.

収録刊行物

キーワード

詳細情報 詳細情報について

  • CRID
    1390001204682973824
  • NII論文ID
    130003815380
  • DOI
    10.2330/joralbiosci1965.38.226
  • ISSN
    03850137
  • 本文言語コード
    ja
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

問題の指摘

ページトップへ