Dual pathways for sweet taste transduction in isolated gerbil taste cells: cAMP and IP3 mediated mechanisms.
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- Uchida Yoshinori
- Department of Physiology, Nagasaki University School of Dentistry
Bibliographic Information
- Other Title
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- スナネズミ味細胞の甘味応答を誘発すると考えられる2つのセカンドメッセンジャー
Abstract
Mechanisms of sweet taste transduction in gerbil taste cells were examined using the conventional whole-cell patch-clamp technique. Outward K+ currents of the taste cell induced by depolarizing electric pulses were reduced by 10 mM Na-saccharin, but were enhanced by amino acid sweeteners of 10 mM D-tryptophan. The outward K+ current was also enhanced by external application of Ca2+ -ionophore, 5μM ionomycin and intracelluar application of 5μM IP3, (inositol-1, 4, 5-trisphosphate). These results suggest that the sweet taste transduction mechanism for Na-saccharin is concerned with an increase of the intracellular cAMP level, while that for D-tryptophan is concerned with an increase of the intracellular IP3, level causing Ca2+ release from the internal stores. Gurmarin, an inhibitor of sweet taste. antagonized the suppressive effect of Na-saccharin on outward K+ currents, but did not affect the enhancing effect of Dtryptophan on outward K+ currents. The results from treatment with gurmarin suggest that the receptor site for Na-saccharin is different from that for D-tryptophan.
Journal
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- Japanese Journal of Oral Biology
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Japanese Journal of Oral Biology 38 (2), 226-234, 1996
Japanese Association for Oral Biology
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Details 詳細情報について
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- CRID
- 1390001204682973824
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- NII Article ID
- 130003815380
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- ISSN
- 03850137
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed