Evaluation of a New <I>S. mutans</I> Bacteria Inspection Kit using PCR Method

DOI 15 References
  • Kariya Naoyuki
    Division of Global Health and Environmental Science, Department of Behavioral Pediatric Dentistry, Okayama University, Graduate School of Medicine and Dentistry
  • Matsumura Seishi
    Division of Global Health and Environmental Science, Department of Behavioral Pediatric Dentistry, Okayama University, Graduate School of Medicine and Dentistry
  • Rodis Omar M M
    Division of Global Health and Environmental Science, Department of Behavioral Pediatric Dentistry, Okayama University, Graduate School of Medicine and Dentistry
  • Ji Ying
    Division of Global Health and Environmental Science, Department of Behavioral Pediatric Dentistry, Okayama University, Graduate School of Medicine and Dentistry
  • Du Xiaopei
    Division of Global Health and Environmental Science, Department of Behavioral Pediatric Dentistry, Okayama University, Graduate School of Medicine and Dentistry
  • Shino Ayako
    Okayama University Hospital of Medicine and Dentistry
  • Shimono Tsutomu
    Division of Global Health and Environmental Science, Department of Behavioral Pediatric Dentistry, Okayama University, Graduate School of Medicine and Dentistry

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Other Title
  • 新しい<I>S. mutans</I>菌検査キットのPCR法を用いた臨床的有用性評価

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Abstract

Proper oral health care is important for the prevention of oral disease, and it is necessary to teach appropriate oral hygiene as early as possible.<BR>Our purpose was to evaluate the effectiveness of an S. mutans measurement kit known as the“Oral Tester (Mutans)”.<BR>52 4-5 year-old nursery school children in Okayama participated in this study. Plaque sampling and analysis using the“Cariostat”and“Oral Tester (Mutans)”was performed by one dentist. The PCR method from prepared DNA was performed and confirmed by electrophoresis.<BR>PCR detection of S. mutans in the high-risk samples of the Oral Tester was 95.5% while it was 50% in the low risk samples.<BR>A significant difference (P<0.001) was seen between the high and low results of The Oral Tester (Mutans) and for the PCR detection of S. mutans.<BR>PCR detection of S. mutans in the high-risk samples of the Cariostat was 87.1% while it was 42.9% in the low-risk samples.<BR>A significant difference (P<0.01) was seen between the high and low results of Cariostat and the PCR detection of S. mutans.<BR>PCR detection of S. sobrinus in the high-risk samples of the Oral Tester (Mutans) was 36.4%while it was 20% in the low risk samples.<BR>PCR detection of S. sobrinus in the high-risk samples of Cariostat was 35.5% while it was 14.3%in the low-risk samples.<BR>There was no statistical significance seen between the high and low risk samples for both the Oral Tester (Mutans) and Cariostat.<BR>The specificity of the Oral Tester (Mutans) in detecting the presence of S. mutans was confirmed by PCR detection and it is therefore useful for caries risk assessment.

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