Assay of the Concentration of Chromogranin A as Stress Indicator in Children's Saliva

DOI 6 Citations 24 References
  • Mitsuhata Chieko
    Department of Pediatric Dentistry, Graduate School of Biomedical Sciences Hiroshima University
  • Suzuki Junji
    Department of Pediatric Dentistry, Graduate School of Biomedical Sciences Hiroshima University
  • Makihira Mika
    Department of Pediatric Dentistry, Graduate School of Biomedical Sciences Hiroshima University
  • Soda Yoshiko
    Department of Pediatric Dentistry, Graduate School of Biomedical Sciences Hiroshima University
  • Kozai Katsuyuki
    Department of Pediatric Dentistry, Graduate School of Biomedical Sciences Hiroshima University

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  • 小児唾液からのストレス指標物質クロモグラニンAの定量

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Abstract

It is well known that dental treatment can induce stress in children. This stress can lead to dental anxiety or fear of future dental care., it is therefore, important to know whether children are under a high degree of stress or not during dental treatment. It was reported that saliva Chromogranin A (CgA) might be a sensitive and promising index for psychosomatic stress in adults. The aim of this study was to verify whether saliva CgA may also be used as an index of stress in children. The subjects were 42 children ranging from 5 to 9 years of age (mean 7.5 years of age). Saliva samples were collected before and after each dental treatment by using a cotton roll. The CgA levels were determined using EIA. In order to determine the level of dental anxiety of the children and their personality traits, we used the Dental Sub-scale of Children Fear Survey Schedule (CFSS-DS) and a questionnaire that included a personality test. The CgA value was measured to be 3.17+/-22.17 pmol/mg protein at pre-treatment and 1.97 +/-1.79 pmol/mg protein post-treatment. Although the value of CgA of 83.8% of the children decreased, there were no significant correlations between CgA values and CFSS-DS. However, children with a high score on the CFSS-DS tended to have a higher CgA value. These results suggest that CgA may be used to verify child stress during dental treatment.

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