Hybridoma and Atherosclerosis

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  • TSUKADA Toyohiro
    Department of Internal Medicine, Tokyo Medical and Dental University
  • KUROIWA Tomoe
    Department of Internal Medicine, Tokyo Medical and Dental University
  • HIROSE Shin
    Department of Internal Medicine, Tokyo Medical and Dental University
  • YAJIMA Michiyoshi
    Department of Internal Medicine, Tokyo Medical and Dental University
  • NUMANO Fujio
    Department of Internal Medicine, Tokyo Medical and Dental University
  • LEE K. T.
    Department of Pathology, Albany Medical College
  • TSUTSUI Masato
    Department of Dermatology, University of Miami
  • ADACHI Kenji
    Department of Dermatology, University of Miami

Bibliographic Information

Other Title
  • HybridomaとAtherosclerosis (第1報)
  • ―Hybri-clonal macrophage antibody (#75)による粥腫内macrophage検索の試み―
  • -Part I: Identification of Macrophage-Derived Foam Cells by Hybri-Clonal Antibody-

Abstract

As the role of macrophage-derived foam cells seems to relate to the progression and regression of atherosclerosis, we attempted to acquire monoclonal antibodies of macrophages, using a hybridoma technique. Use of these antibodies enables identification of macrophage-derived foam cells in atherosclerotic lesions.<br>Using BALB/C and SP2/O mice, we established several clones of mouse hybridoma, which produce monoclonal antibodies against pig alveolar macrophages. #75 antibody obtained in this series was confirmed to be specific for macrophages. With this antibody, we studied atherosclerotic lesions in aorta of pigs fed a one percent cholesterol diet for 20 weeks, using an indirect immunoperoxidase procedure. Numerous foam cells located in fibrous caps or immediately under the fibrous cap as well as the necrotic foci exhibited a positive reaction.<br>Thus, the use of hybri-clonal antibody to determine the role of macrophage-derived foam cells in atherosclerotic lesions should yield pertinent data related to the lesions seen in atherosclerosis and related disorders.

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