Analysis of LDH isoenzyme patterns in cell lines of the small cell carcinoma (lung) and retinoblastoma.

DOI

Bibliographic Information

Other Title
  • 肺小細胞がんおよび網膜芽細胞しゅ培養株のＬＤＨアイソエンザイムパターンの検討

Abstract

The LDH isoenzyme pattern of human lung cancer and retinoblastoma cell lines was studied by enzyme cytochemical and electrophoretical procedures. Seven small cell carcinoma, two adenocarcinoma and two retinoblastoma cell lines were used in this study. By the urea inhibition test of LDH enzyme cytochemistry, none of small cell carcinoma and retinoblastoma cell lines showed any decrease of LDH staining. However, the intensity of LDH stain decreased in both cell lines of adenocarcinoma by the urea inhibition test. By the electrophoretical analysis of LDH isoenzyme pattern both small cell carcinoma and retinoblastoma cell lines showed almost the same LDH isoenzyme pattern with the peak of LDH II or III, and their M/H ratio of LDH was 0.86±0.16 and 0.92±0.15, respectively. On the other hand, the adenocarcinoma cell line revealed high activity of LDH IV and V, and subsequently showed increased M/H ratio of LDH. In short, the LDH isoenzyme pattern of small cell carcinoma is very similar to that of retinoblastoma rather than that of adenocarcinoma. Therefore, these results may indicate a common biological nature between small cell carcinoma and retinoblastoma as a member of neuroendocrine tumors.

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Details 詳細情報について

  • CRID
    1390001204690578304
  • NII Article ID
    130003925621
  • DOI
    10.5795/jjscc.24.451
  • ISSN
    18827233
    03871193
  • Text Lang
    ja
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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