書誌事項
- タイトル別名
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- Fundamental study detecting apoptosis by the TUNEL(TdT-mediated d-UTP nick end-labeling) method in cytology preparations.
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抄録
We report a fundamental study detecting apoptosis (AP) by the TdT-mediated d-UTP nick end-labeling (TUNEL) method in cytology preparations of a human lymphocyte established cell line (C 5/TK 1), ascites fluid from patients with or without malignancies, and imprint or squash preparations of tissue samples. There is no distinct difference between the conditions using 95% ethanol and 100% methanol as a fixative for routine fixation and AP could be demonstrated even in the cytology preparations which were stored in the fixatives for up to 10 months. On the other hand, prolonged fixation in a formaldehyde fixative (20% formalin) caused nonspecific labeling, and it seemed to be inappropriate for long-time storage. Proteinase K (PK) pretreatment was required for all the fixatives used, and incubation with 10 μg of PK per ml for 15 minutes at room temperature always gave satisfactory results, though incubation with 20 μg of PK per ml or higher concentrations produced cell detachment due to overdigestion. Morphological analysis under light microscopical observation, and comparison of the localization and distribution of AP in cell preparations with those of tissue samples confirmed the reliability of AP detection by the TUNEL method in cell preparations.
収録刊行物
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- 日本臨床細胞学会雑誌
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日本臨床細胞学会雑誌 36 (4), 358-363, 1997
公益社団法人 日本臨床細胞学会
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詳細情報 詳細情報について
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- CRID
- 1390001204691896320
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- NII論文ID
- 130003926383
- 110001227917
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- NII書誌ID
- AN00198721
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- ISSN
- 18827233
- 03871193
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可