The in situ hybridization (ISH) method for detecting HPV in cytodiagnosis. A study of fixation and processing methods.

DOI 3 Citations
  • KINA Katsunari
    Department of Clinical Labolatory, Juntendo Urayasu Hospital, Juntendo University, School of Medicine
  • ISHI Kazuhisa
    Department of Clinical Labolatory, Juntendo Urayasu Hospital, Juntendo University, School of Medicine
  • KAZAMA Reiko
    Department of Clinical Labolatory, Juntendo Urayasu Hospital, Juntendo University, School of Medicine
  • KOYATSU Junichi
    Department of Clinical Labolatory, Juntendo Urayasu Hospital, Juntendo University, School of Medicine
  • KAWASHIMA Toru
    Department of Clinical Labolatory, Juntendo Urayasu Hospital, Juntendo University, School of Medicine
  • SAITOH Akira
    Department of Clinical Labolatory, Juntendo Urayasu Hospital, Juntendo University, School of Medicine
  • NAKAMURA Haruji
    TORAY Basic Institute

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Other Title
  • 細胞診におけるＨＰＶ　ＤＮＡ検出のためのＩｎ　ｓｉｔｕ　ｈｙｂｒｉｄｉｚａｔｉｏｎ（ＩＳＨ）法　固定法，処理法の検討
  • A study of fixation and processing methods
  • 固定法, 処理法の検討

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Using the ISH method with the objective of detecting HPV DNA in cytodiagnosis of the uterine cervix, we studied methods fixation and processing (proteopeptic response and their combinations) and obtained the following results.<BR>1) When ISH operational techniques were used in normal tissue specimens, staining was better with 15% neutral buffered formalin fixation than 95% ethanol, acetone fixation.<BR>2) When 95% ethanol fixation was used, it was possible to omit the proteopeptic reaction included in the ISH operational techniques, and favorable staining results were obtained. This indicates that, during normal cytodiagnosis it is not necessary to prepare a special fixation solution.<BR>3) From Papanicolaou staining with restaining specimens, HPV-DNA positive results were obtained. Given the above results, it is comsidered possible to conduct retrospective studies of HPV from Papanicolaou staining with restaining specimens in cytodiagnosis of the uterine cervix.

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