腹水カイロミクロンの分析

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  • Analysis of Ascites Chylomicrons

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A patient with nephrotic syndrome and morbus Kimura (eosinophilic granuloma) showed chylous ascites. Ascites chylomicron was analyzed and further subjected to study the substrate specificity of lipoprotein lipase and hepatic triglyceride lipase. Ascites triglyceride and cholesterol concentrations were 191 ans 12mg/dl, respectively. Both apo CII and apo CIII contents in ascites were approximately one-third of those of plasma from normal subjects. Ascites chylomicron was incubated with either lipoprotein lipase or hepatic triglyceride lipase, which were prepared from human postheparin plasma using heparin-Sepharose affinity chromatography. Lipoprotein lipase hydrolyzed ascites chylomicron, while hepatic triglyceride lipase was unable to do that. These results suggest different functions of these two lipases in chylomicron catabolism.

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