Application of the Ultrafast papanicolaou stain to airdried smears in intraoperative diagnosis.
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- MINATO Hiroshi
- Pathology Section, Kanazawa University Hospital
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- HORITA Tomoko
- Pathology Section, Kanazawa University Hospital
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- HISATOMI Motoji
- Pathology Section, Kanazawa University Hospital
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- NONOMURA Akitaka
- Pathology Section, Kanazawa University Hospital
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- OHTA Kouichi
- Department of Pathology, Ishikawa Prefectural Central Hospital
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- MATSUOKA Katsuyu
- Department of Pathology, Ishikawa Prefectural Central Hospital
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- MIYAMOTO Makiko
- Department of Pathology, Ishikawa Prefectural Central Hospital
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- KURUMAYA Hiroshi
- Department of Pathology, Ishikawa Prefectural Central Hospital
Bibliographic Information
- Other Title
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- 乾燥迅速パパニコロウ染色の術中診断への応用
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Abstract
Objective: We assessed the usefulness of the Ultrafast Papanicolaou stain (UFP), incorporating air-dried rehydration in intraoperative diagnosis.<BR>Study Design: This study involved 482 randomly selected surgical specimens for intraoperative consultation (32 organs from the systemic body: 311 benign and 171 malignant) and 51 cases of peritoneal washing fluid. Specimens were prepared for UFP, frozen sections, Papanicolaou (Pap.), Giemsa, and Alcian-blue stain. Each was diagnosed intraoperatively based on UFP and frozen sections, and postoperatively based on Pap. and permanent histological sections.<BR>Results: UFP is simple and fast and produced results within 2 minutes. UFP color differed somewhat from that of Pap. but retained transparency with polychromasia. Nuclear features were clearly observed and nucleoli conspicuous. Lysis of erythrocytes resulted in a clear background. Overall UFP accuracy was 97.3%, frozen section 98.2%, and Pap. 96.1%. Final overall accuracy was 99%.<BR>Conclusion: UFP conducted on air-dried smears has many advantages over direct wet fixation and, with adequate sampling for each specimen, is very useful in intraoperative diagnosis.
Journal
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- The Journal of the Japanese Society of Clinical Cytology
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The Journal of the Japanese Society of Clinical Cytology 40 (3), 249-256, 2001
The Japanese Society of Clinical Cytology
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Details 詳細情報について
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- CRID
- 1390001204693232896
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- NII Article ID
- 130003758843
- 10007682668
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- NII Book ID
- AN00198721
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- ISSN
- 18827233
- 03871193
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed