Intestinal Epithelial Cell-specific Deletion of α-Mannosidase II Ameliorates Experimental Colitis

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  • Suzuki Koichiro
    Division of Biochemistry, Faculty of Pharmacy, Keio University Research Fellow of Japan Society for the Promotion of Science
  • Yamada Takahiro
    Division of Biochemistry, Faculty of Pharmacy, Keio University
  • Yamazaki Keiko
    Division of Genomic Epidemiology and Clinical Trials, Clinical Trials Research Center, Nihon University School of Medicine Laboratory for Genotyping Development, RIKEN Center for Integrative Medical Sciences
  • Hirota Masato
    Division of Biochemistry, Faculty of Pharmacy, Keio University
  • Ishihara Narumi
    Division of Biochemistry, Faculty of Pharmacy, Keio University
  • Sakamoto Mizuki
    Division of Biochemistry, Faculty of Pharmacy, Keio University
  • Takahashi Daisuke
    Division of Biochemistry, Faculty of Pharmacy, Keio University
  • Iijima Hideki
    Department of Gastroenterology and Hepatology, Osaka University Graduate School of Medicine
  • Hase Koji
    Division of Biochemistry, Faculty of Pharmacy, Keio University International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo (IMSUT)

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<p>Inflammatory bowel disease (IBD) is a refractory disease of the gastrointestinal tract that is believed to develop in genetically susceptible individuals. Glycosylation, a type of post-translational modification, is involved in the development of a wide range of diseases, including IBD, by modulating the function of various glycoproteins. To identify novel genes contributing to the development of IBD, we analyzed single nucleotide polymorphisms (SNPs) of glycosylation-related genes in IBD patients and identified MAN2A1, encoding alpha-mannosidase II (α-MII), as a candidate gene. α-MII plays a crucial, but not exclusive, role in the maturation of N-glycans. We also observed that intestinal epithelial cells (IECs), which establish the first-line barrier and regulate gut immunity, selectively expressed α-MII with minimal expression of its isozyme, alpha-mannosidase IIx (α-MIIx). This led us to hypothesize that IEC-intrinsic α-MII is implicated in the pathogenesis of IBD. To test this hypothesis, we generated IEC-specific α-MII-deficient (α-MIIΔIEC) mice. Although α-MII deficiency has been shown to have a minimal effect on N-glycan maturation in most cell types due to the compensation by α-MIIx, ablation of α-MII impaired the maturation of N-glycans in IECs. α-MIIΔIEC mice were less susceptible to dextran sulfate sodium-induced colitis compared with control littermates. In accordance with this, neutrophil infiltration in the colonic mucosa was attenuated in α-MIIΔIEC mice. Furthermore, gene expression levels of neutrophil-attracting chemokines were downregulated in the colonic tissue. These results suggest that IEC-intrinsic α-MII promotes intestinal inflammation by facilitating chemokine expression. We propose SNPs in MAN2A1 as a novel genetic factor for IBD.</p><p>Key words: inflammatory bowel disease, alpha-mannosidase II, intestinal epithelial cell, N-glycosylation</p>

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