A Fission Yeast SNAP-25 Homologue, SpSec9, Is Essential for Cytokinesis and Sporulation
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- Nakamura Taro
- Department of Biology, Graduate School of Science, Osaka City University
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- Kashiwazaki Jun
- Department of Biology, Graduate School of Science, Osaka City University
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- Shimoda Chikashi
- Department of Biology, Graduate School of Science, Osaka City University
書誌事項
- タイトル別名
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- Fission Yeast Snap 25 Homologue SpSec9 Is Essential for Cytokinesis and Sporulation
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抄録
The soluble NSF attachment protein 25 (SNAP-25) is a component of the SNARE complex that is essential for regulated exocytosis in diverse cell types. Here, we identified a fission yeast SNAP-25 homologue, SpSec9. The sec9+ gene was essential for vegetative growth. sec9 mRNA was detected in vegetative cells and further increased during sporulation. This increase during sporulation was dependent on Mei4, a meiosis-specific transcription factor. A sporulation-deficient sec9 mutant was isolated by random PCR mutagenesis (sec9-10). The sec9-10 mutant also exhibited temperature sensitivity for growth and cell division was found to arrest before completion of cell separation at restrictive temperatures. In sec9-10 cells, the forespore membrane was normally initiated near spindle pole bodies during meiosis II. However, subsequent extension of the membrane was severely impaired. These results indicate that SpSec9 plays an important role both in cytokinesis and in sporulation.<br>
収録刊行物
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- Cell Structure and Function
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Cell Structure and Function 30 (2), 15-24, 2005
日本細胞生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001204694930048
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- NII論文ID
- 130004137494
- 40007227356
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- NII書誌ID
- AA0060007X
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- COI
- 1:STN:280:DC%2BD2Mrpt1Gntw%3D%3D
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- ISSN
- 13473700
- 03867196
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- NDL書誌ID
- 7889087
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- PubMed
- 16272747
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可