Filamin B Enhances the Invasiveness of Cancer Cells into 3D Collagen Matrices

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  • Iguchi Yuta
    Faculty of Advanced Life Science, Hokkaido University
  • Ishihara Seiichiro
    Faculty of Advanced Life Science, Hokkaido University Research Center for Cooperative Projects, Graduate School of Medicine, Hokkaido University
  • Uchida Yoshimi
    Faculty of Advanced Life Science, Hokkaido University
  • Tajima Kaori
    Faculty of Advanced Life Science, Hokkaido University
  • Mizutani Takeomi
    Faculty of Advanced Life Science, Hokkaido University
  • Kawabata Kazushige
    Faculty of Advanced Life Science, Hokkaido University
  • Haga Hisashi
    Faculty of Advanced Life Science, Hokkaido University Research Center for Cooperative Projects, Graduate School of Medicine, Hokkaido University

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  • Enhances the invasiveness of cancer cells into 3D collagen matrices

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Abstract

Numerous types of cancer cells migrate into extracellular tissues. This phenomenon is termed invasion, and is associated with poor prognosis in cancer patients. In this study, we demonstrated that filamin B (FLNb), an actin-binding protein, is highly expressed in cancer cell lines that exhibit high invasiveness, with a spindle morphology, into 3D collagen matrices. In addition, we determined that knockdown of FLNb in invasive cancer cells converts cell morphology from spindle-shaped, which is associated with high invasiveness, to round-shaped with low invasiveness. Furthermore, di-phosphorylation of myosin regulatory light chain (MRLC) and phosphorylation of focal adhesion kinase (FAK) are inhibited in FLNb-knockdown cancer cells. These results suggest that FLNb enhances invasion of cancer cells through phosphorylation of MRLC and FAK. Therefore, FLNb may be a new therapeutic target for invasive cancers.

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