Expression analysis of obg, the gene controlling morphological development of Streptomyces griseus IFO 13189.
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- Okamoto Susumu
- National Food Research Institute
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- Okamoto-Hosoya Yoshiko
- National Food Research Institute
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- Ochi Kozo
- National Food Research Institute
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説明
The expression of the obg gene of S.griseus, which codes for a GTP-binding protein and controls morphological development of this organism, was studied by Western and Northern blot analyses. Western analysis revealed that Obg protein level sharply decreases just after the onset of aerial mycelium development or at the end of vegetative growth on agar plate cultures, indicating that expression of obg is regulated in a growth phase-dependent manner. The obg gene was found to be transcribed as a mRNA of 1.7 kb and its transcription was cut off just prior to the decrease of Obg protein, indicating that obg gene expression is controlled at the transcription level.
収録刊行物
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- 日本放線菌学会誌
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日本放線菌学会誌 14 (1), 17-21, 2000
日本放線菌学会
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詳細情報 詳細情報について
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- CRID
- 1390001204700137088
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- NII論文ID
- 130004487505
- 10008260984
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- NII書誌ID
- AN10080091
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- COI
- 1:CAS:528:DC%2BD3cXmsF2mtbw%3D
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- ISSN
- 18816371
- 09145818
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- NDL書誌ID
- 5447373
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDLサーチ
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- CiNii Articles
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- 使用不可