Efficient Transgene Expression in Chrysanthemum, <i>Chrysanthemum morifolium</i> Ramat., with the Promoter of a Gene for Tobacco Elongation Factor 1 α Protein

DOI 4 Citations Open Access
  • AIDA Ryutaro
    Department of Genetics and Physiology, National Institute of Floricultural Science
  • NAGAYA Shingo
    Graduate School of Biological Sciences, Nara Institute of Science and Technology
  • YOSHIDA Kazuya
    Graduate School of Biological Sciences, Nara Institute of Science and Technology
  • KISHIMOTO Sanae
    Department of Genetics and Physiology, National Institute of Floricultural Science
  • SHIBATA Michio
    Department of Genetics and Physiology, National Institute of Floricultural Science
  • OHMIYA Akemi
    Department of Genetics and Physiology, National Institute of Floricultural Science

Bibliographic Information

Other Title
  • Efficient Transgene Expression in Chrysanthemum, Chrysanthemum morifolium Ramat., with the Promoter of a Gene for Tobacco Elongation Factor 1 α Protein

Description

We investigated the usefulness of the promoter of a gene for tobacco elongation factor 1α protein (EF1 α) for transgene expression in the chrysanthemum (Chrysanthemum morifolium Ramat.). The EF1 α promoter was fused to the β-glucuronidase gene (gus) and introduced into the chrysanthemum. We obtained 238 putative transformants and GUS assay of the leaves of the in vitro plants revealed that 29.0% (69/238) of the putative plants were GUS-positive. The plants in the greenhouse that were 20 months after regeneration still showed a GUS activity in their leaves and petals. The tobacco EF1 α promoter expressed the transgene more efficiently than the 35S promoter of Cauliflower mosaic virus and could be used for transgene expression in chrysanthemum.

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Details 詳細情報について

  • CRID
    1390001204701002752
  • NII Article ID
    130003396714
  • DOI
    10.6090/jarq.39.269
  • COI
    1:CAS:528:DC%2BD2sXhvFKnsr0%3D
  • ISSN
    21858896
    00213551
    http://id.crossref.org/issn/00213551
  • Text Lang
    en
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
    • OpenAIRE
  • Abstract License Flag
    Disallowed

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