Species Diversity of Nocardiae Isolated from Lake and Moat Sediment Samples

  • Yamamura Hideki
    Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi
  • Hayakawa Masayuki
    Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi
  • Nakagawa Youji
    Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi
  • Iimura Yuzuru
    Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi

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説明

We investigated the taxonomic diversity of Nocardia strains freshly isolated from six sediment samples taken from Lake Suwa, Nagano Prefecture, and from the moat surrounding Takeda Shrine, Yamanashi Prefecture, Japan. A restriction fragment length polymorphism (RFLP) analysis of 16S rDNA was used for species-level identification. Of the 25 test isolates, 23 strains were identified as N. asteroides, N. carnea, N. flavorosea, N. nova, N. uniformis, or identified as belonging to the N. africana-N. veterana group. Based on RFLP patterns, the remaining two strains were differentiated from the known Nocardia species.

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