Cloning System for Streptomyces kasugaensis Using the Hybrid Melanin-Synthesizing Gene melE as a Chromogenic Marker
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- Kojima Ikuo
- Department of Biotechnology, Akita Prefectural University
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- Kasuga Kano
- Department of Biotechnology, Akita Prefectural University
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- Kobayashi Masayuki
- Department of Biotechnology, Akita Prefectural University
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- Matsuo Masanao
- Diagnostics Research Laboratories, Daiichi Pure Chemicals Co., Ltd.
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- Akagawa Hisayoshi
- Department of Bioactive Molecules, National Institute of Infectious Diseases
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- Mizuno Satoshi
- Department of Bioactive Molecules, National Institute of Infectious Diseases
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Description
Streptomyces kasugaensis, a producer of kasugamycin, is listed as a particularly safe Streptomyces host in the Japan Guidelines for Recombinant DNA Experiments. In search of a chromogenic selectable marker usable for S. kasugaensis, melanin-synthesizing melE was constructed by placement of the operon for the melanin-synthesizing gene (melC) from Streptomyces antibioticus under the control of the promoter of the erythromycin-resistance gene (ermE) from Saccharopolyspora erythraea. Combined use of melE and the thiostrepton-resistance gene from Streptomyces azureus yielded vectors pSK216 and pSK221, derivatives of pSK2 from S. kasugaensis MB273. In S. kasugaensis and Streptomyces lividans, melE-harboring pSK216 and pSK221 induced detectable amounts of melanin pigment on agar media in marked contrast to pSK2-based pSK212 carrying melC, thereby establishing an efficient cloning system for S. kasugaensis.
Journal
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- Actinomycetologica
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Actinomycetologica 17 (2), 29-32, 2003
The Society for Actinomycetes Japan
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Details 詳細情報について
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- CRID
- 1390001204701345792
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- NII Article ID
- 130004487528
- 10012501124
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- NII Book ID
- AN10080091
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- COI
- 1:CAS:528:DC%2BD2cXktVOku7s%3D
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- ISSN
- 18816371
- 09145818
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- NDL BIB ID
- 6876626
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed