Isolation and characterization of antibacterial substances produced by marine actinomycetes in the presence of seawater

DOI Web Site 被引用文献1件 参考文献27件
  • Imada Chiaki
    Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology
  • Koseki Naoko
    Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology
  • Kamata Masazumi
    Department of Science of Aesthetics and Art, Yamano College of Aesthetics
  • Kobayashi Takeshi
    Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology
  • Hamada-Sato Naoko
    Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology

この論文をさがす

抄録

Actinomycetes, which produced antibacterial substances against Gram-positive bacteria in the presence of seawater only, were isolated from marine environment and characterized. Of the 100 isolates from Otsuchi Bay in Iwate, 41 failed to produce any antibacterial substances in the absence of seawater. However, of the 41, two were found to produce antibacterial substances in the presence of seawater. Strain No. 18 exhibited higher activity than strain No. 28 and was, therefore, selected for further studies. Phylogenetic analysis and physiological characterization showed a high similarity between strain No. 18 and Micromonospora globosa. However, M. globosa JCM 3126 did not produce any antibiotics in the presence of seawater. Therefore, the effect of seawater on the growth of strain No. 18 and its production of antibacterial substances was investigated. Strain No. 18 grew in a medium prepared with artificial seawater at a concentration range of 0–140% (v/v). The optimal concentration of growth ranged from 10 to 30% (v/v). However, production of antibacterial substances was observed in the concentration range 60–110% (v/v) despite poor growth. Thus, the production of antibacterial substances is seawater-dependent. The purification and characterization of the substances is now in progress.

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (27)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ