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- Ueki Masashi
- Antibiotics Laboratory, Chemical Biology Department, RIKEN ASI
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- Suzuki Ryuichiro
- Antibiotics Laboratory, Chemical Biology Department, RIKEN ASI
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- Takamatsu Satoshi
- Kitasato Institute for Life Sciences, Kitasato University
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- Takagi Hiroshi
- Antibiotics Laboratory, Chemical Biology Department, RIKEN ASI
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- Uramoto Masakazu
- Antibiotics Laboratory, Chemical Biology Department, RIKEN ASI
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- Ikeda Haruo
- Kitasato Institute for Life Sciences, Kitasato University
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- Osada Hiroyuki
- Antibiotics Laboratory, Chemical Biology Department, RIKEN ASI
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抄録
Desferrioxamine B (desferal®) is the only therapeutic agent for chronic iron overload and acute iron intoxication. Therefore, new drug candidates are required for more effective treatments. Recent genome analyses of microorganisms have enabled us to identify cryptic gene clusters of secondary metabolism. In a genomic analysis of Streptomyces avermitilis, we found the putative biosynthetic gene cluster for nocardamin and desferrioxamine derivatives, the production of which by S. avermitilis had been unknown. To determine the synthesis of nocardamin and discover new derivatives in S. avermitilis, we performed a comprehensive analysis and isolation of secondary metabolites of S. avermitilis. We obtained nocardamin and its related compounds, including a derivative that initially was isolated as a microbial product. The biosynthetic pathway of nocardamin and the substrate specificity of its biosynthesis enzymes were proposed based on the structures of the isolated nocardamin derivatives. The production of nocardamin by S. avermitilis was completely suppressed by the addition of more than 5 μM ferric ions. An iron-dependent regulatory protein (IdeR)-binding motif was located upstream of the sidABCD operon, using the profile hidden Markov model method. To determine the regulation of nocardamin synthesis by IdeR in S. avermitilis, we constructed ideR deletion mutants. The production of nocardamin by the deletion mutants was not suppressed by ferric ions (up to 100 μM).
収録刊行物
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- 日本放線菌学会誌
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日本放線菌学会誌 23 (2), 34-39, 2009
日本放線菌学会
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詳細情報 詳細情報について
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- CRID
- 1390001204702624896
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- NII論文ID
- 10025872918
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- NII書誌ID
- AN10080091
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- COI
- 1:CAS:528:DC%2BC3cXjvF2nsbY%3D
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- ISSN
- 18816371
- 09145818
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- NDL書誌ID
- 10530275
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 抄録ライセンスフラグ
- 使用不可